Welcome to protoSpaceJAM’s documentation!

protoSpaceJAM is a CRISPR knock-in design tool that designs gRNAs and HDR donors that maximizes success rate using an array of known rules.

Workflow overview

Check out the Usage section for more information. Python Source code is available.

Contents

• Usage
  • Basic usage
  • Create a submission list
  • Load an example submission list
  • Confirm submission list
  • Launch protoSpaceJAM
  • View/Download results
• Algorithm
  • Overview
  • gRNA scoring
  • Recoding strategy
  • DNA donor processing strategy
• Source code
• Changelog
  • Algorithm
  • Portal